Volume No. :   2

Issue No. :  1

Year :  2011

ISSN Print :  0976-2973

ISSN Online :  2321-581X


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Statistical Optimization of Lipase Production from Bacillus spp by Central Composite Design



Address:   R.Saranya1, K. Tamilarasan1, N. Peatciyammal1, Dr. Rajamaniccam L. Sai2, Dr. M. Dharmendira Kumar2*
1Department of Biotechnology, Madha Engineering College, Chennai 600069, INDIA
2Department of Chemical Engineering, Alagappa College of Technology, Anna University, Chennai 600025, INDIA
*Corresponding Author
DOI No:

ABSTRACT:
A Response Surface approach has been used to study the production of an extracellular lipase from Bacillus species. This research paper mainly focused on developing a media component and physical parameters for lipase production by Central Composite Design (CCD).Interaction were studied for four different factors are olive oil concentration, ammonium sulphate concentration, pH and Temperature. By using the statistical approach (Response Surface Methodology) the optimum enzyme activity of 8.69 IU/ml was predicted when olive oil concentration 1.5%, ammonium sulphate concentration 0.4%, pH 8, Temperature 40ÂșC.
KEYWORDS:
Central Composite Design, Lipase activity, Maximum yield, Response Surface Methodology, Statistical approach
Cite:
R.Saranya, K. Tamilarasan, N. Peatciyammal, Rajamaniccam L. Sai, M. Dharmendira Kumar. Statistical Optimization of Lipase Production from Bacillus spp by Central Composite Design. Research J. Engineering and Tech. 2(1): Jan.-Mar. 2011 page 31-35.
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