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Research Journal of Engineering and Technology
ISSN: 2321-581X(Online), 0976-2973(Print)
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Optimal Production and Purification of Citric Acid from Cane Molasses by Aspergillus niger MTCC 282 using Response Surface Methodology
M. Anil Kumar
Deena Durairaj, M. Anil Kumar and M. Seenuvasan*
Department of Biotechnology, Madha Engineering College, Chennai- 600 069, India.
A sequential optimization based on statistiscal design was employed to optimize the media constituents for the improvement of citric acid production from cane molasses through surface fermentation using Aspergillus niger MTCC 282. The selected medium constituents were optimized by the central composite design under the response surface methodology where the maximum yield of citric acid and minimal residual sugar concentration was found to be 28.75 g/L and 0.9 g/L at pH at a temperature of 30ºC having a substrate concentration of 120 g/L under 9 days of incubation. The produced citric acid was then separated using cation-exchange chromatography.
Citric acid, Residual sugar, Aspergillus niger, RSM.
Deena Durairaj, M. Anil Kumar , M. Seenuvasan. Optimal Production and Purification of Citric Acid from Cane Molasses by Aspergillus niger MTCC 282 using Response Surface Methodology. Research J. Engineering and Tech. 2(1): Jan.-Mar. 2011 page 36-41.
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