Author(s): T. Uma, V.N. Saranya, M. Anil Kumar, S. Selvanaveen, R. Viveka, M. Seenuvasan

Email(s): seenuchem786@gmail.com

DOI: Not Available

Address: T. Uma, V.N. Saranya, M. Anil Kumar, S. Selvanaveen, R. Viveka and M. Seenuvasan*
1Department of Biotechnology, Madha Engineering College, Chennai. India.
*Corresponding Author

Published In:   Volume - 2,      Issue - 3,     Year - 2011


ABSTRACT:
The extra cellular lipase produced by a novel strain of Bacillus sp. isolated from soil was used as an alternative for commercially available detergents for removing stains like oil, grease and blood stains in fabric clothes, as lipase shows the effect of hydrolysis of fat. The production was carried out by submerged fermentation using olive oil as substrate. The production media was optimized using the Response Surface Methodology (RSM) to get maximum yield with at most lipase activity. Thus Lipase produced had highest activity at pH 8, 30?C under incubation period of 24 h. The dialyzed sample of the partially purified enzyme was used to determine the molecular weight of lipase using sodium do-decyl sulphate poly acryl amide gel electrophoresis, which was 95 kDa. The stability of the enzyme was studied inferring it with maximum activity at pH 8.0 and temperature of 30?C. The detergent action of the enzyme was analyzed using the ultra filtered enzyme samples. The stain removal capacity of the lipase was better than the commercial detergents used.


Cite this article:
T. Uma, V.N. Saranya, M. Anil Kumar, S. Selvanaveen, R. Viveka , M. Seenuvasan. Optimal Production, Purification and Characterization of Extracellular Lipase by an Isolated Bacillus Sp. and It’s Ability as a Bio-Detergent. Research J. Engineering and Tech. 2(2): April-June 2011 page146-151.


Recomonded Articles:

Author(s): D.Vasudevan, P.Balashanmugam

DOI:         Access: Open Access Read More

Author(s): Suchita Rai, K. L. Wasewar, M. J. Chaddha, J. Mukhopadhyay

DOI:         Access: Open Access Read More

Author(s): Sony Salot, Shankar Sehgal, B.S Pabla, Harmesh Kumar

DOI: 10.5958/2321-581X.2017.00048.4         Access: Open Access Read More

Author(s): Piyush M. Kale, S. T. Bagde

DOI:         Access: Open Access Read More

Author(s): S. S. K. Deepak

DOI:         Access: Open Access Read More

Author(s): Nitika Goyal, Deepam Goyal

DOI: 10.5958/2321-581X.2017.00012.5         Access: Open Access Read More

Author(s): Archit Roy, Vinit Shahdeo, Rajesh Kaluri

DOI: 10.5958/2321-581X.2019.00004.7         Access: Closed Access Read More

Author(s): Parakkal Rajendran Dignesh, Suriya Krishnan, Valarmathi Rajasekaran, Balaraman Kumar

DOI:         Access: Open Access Read More

Author(s): S. S. Yattinahalli, S. B. Kapatkar, S.N.Mathad

DOI: 10.5958/2321-581X.2016.00024.6         Access: Open Access Read More

Author(s): Muthukumaran P*, T. Paramasivan, M. Priya, S. Abarna, P. Ramalingam and N. Saraswathy

DOI: 10.5958/2321-581X.2017.00006.X         Access: Open Access Read More

Author(s): Harkamal Preet Singh

DOI: 10.5958/2321-581X.2016.00030.1         Access: Open Access Read More

Author(s): Gurpreet Singh, Harkamal Preet Singh, Vinay Arora

DOI: 10.5958/2321-581X.2017.00005.8         Access: Open Access Read More

Author(s): Dibyendu Bikash Datta, Shashanka Shankar Boruah

DOI: 10.5958/2321-581X.2017.00007.1         Access: Open Access Read More

Author(s): R. K. Pal

DOI: 10.5958/2321-581X.2015.00068.9         Access: Open Access Read More

Author(s): Jay Patel, Preet Mota, Akshay Salvi, Prerna Goswami, M. A. K Kerawala

DOI: 10.5958/2321-581X.2017.00025.3         Access: Open Access Read More

Author(s): Arjun K Sunil, Anil Mahwar

DOI: 10.5958/2321-581X.2016.00029.5         Access: Open Access Read More

Author(s): Love Verma, Ram Nivas Giri

DOI:         Access: Open Access Read More

Author(s): Veerabhuvaneshwari Veerichetty, Baby Shalini M, Balaji Sadhasivama, Saraswathy Nachimuthu

DOI: 10.5958/2321-581X.2017.00067.8         Access: Open Access Read More

Research Journal of Engineering and Technology (RJET) is an international, peer-reviewed, research journal aiming at promoting and publishing original high quality research in all disciplines of engineering sciences and technology....... Read more >>>

RNI: Not Available                     
DOI: 10.5958/2321-581X 


Recent Articles




Tags