Volume No. :   2

Issue No. :  3

Year :  2011

ISSN Print :  0976-2973

ISSN Online :  2321-581X


Registration

Allready Registrered
Click to Login

Optimal Production, Purification and Characterization of Extracellular Lipase by an Isolated Bacillus Sp. and It’s Ability as a Bio-Detergent



Address:   T. Uma, V.N. Saranya, M. Anil Kumar, S. Selvanaveen, R. Viveka and M. Seenuvasan*
1Department of Biotechnology, Madha Engineering College, Chennai. India.
*Corresponding Author
DOI No:

ABSTRACT:
The extra cellular lipase produced by a novel strain of Bacillus sp. isolated from soil was used as an alternative for commercially available detergents for removing stains like oil, grease and blood stains in fabric clothes, as lipase shows the effect of hydrolysis of fat. The production was carried out by submerged fermentation using olive oil as substrate. The production media was optimized using the Response Surface Methodology (RSM) to get maximum yield with at most lipase activity. Thus Lipase produced had highest activity at pH 8, 30?C under incubation period of 24 h. The dialyzed sample of the partially purified enzyme was used to determine the molecular weight of lipase using sodium do-decyl sulphate poly acryl amide gel electrophoresis, which was 95 kDa. The stability of the enzyme was studied inferring it with maximum activity at pH 8.0 and temperature of 30?C. The detergent action of the enzyme was analyzed using the ultra filtered enzyme samples. The stain removal capacity of the lipase was better than the commercial detergents used.
KEYWORDS:
Bacillus sp., Lipase, activity, pH, Temperature, RSM.
Cite:
T. Uma, V.N. Saranya, M. Anil Kumar, S. Selvanaveen, R. Viveka , M. Seenuvasan. Optimal Production, Purification and Characterization of Extracellular Lipase by an Isolated Bacillus Sp. and It’s Ability as a Bio-Detergent. Research J. Engineering and Tech. 2(2): April-June 2011 page146-151.
[View HTML]      [View PDF]



Visitor's No. :   160101