Volume No. :   4

Issue No. :  4

Year :  2013

ISSN Print :  0976-2973

ISSN Online :  2321-581X


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Statistical Optimization of Alkaline Levan Production using Bacillus subtilis



Address:   J. Niranjan, Fazal Basha, G. Suganya, M. Anil Kumar, M. Seenuvasan, S. Selvanaveen*
Department of Biotechnology, Madha Engineering College, Chennai.
*Corresponding Author
DOI No:

ABSTRACT:
The industrially used biopolymer levan as an emulsifying agent was produced in large amounts by optimizing the physical parameters like pH, temperature and incubation time, with the central composite design under the response surface methodology where the maximum yield of levan was found to be 268 µg/ml at a pH of 8.0, a temperature of 20°C and the incubation period of 24th h. The pH- and thermal stabilities of levan-forming activity were studied and arrived at the maximum activity at pH 7.0 and at 20°C respectively, thereby inferring that the levan is optimally produced by Bacillus subtilis in an alkaline environment. Scanning electron microscope was used to examine the surface morphology levan-producing Bacillus subtilis that showed the levan polymer as white dust-like particles.
KEYWORDS:
Bacillus subtilis, Levan polymer, RSM, Scanning electron microscope
Cite:
J. Niranjan, Fazal Basha, G. Suganya, M. Anil Kumar, M. Seenuvasan, S. Selvanaveen. Statistical Optimization of Alkaline Levan Production using Bacillus subtilis. Research J. Engineering and Tech. 4(4): Oct.-Dec., 2013 page 169-173.
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