Author(s): Dandayudham Ramya, Kaushik Raman Varsha, Venkatesh Ranjitha, P. Senthil Kumar, Vaidyanathan Vinoth Kumar

Email(s): vinothkumar.v@ktr.srmuniv.ac.in

DOI: Not Available

Address: Dandayudham Ramya1, Kaushik Raman Varsha1, Venkatesh Ranjitha, P. Senthil Kumar2, Vaidyanathan Vinoth Kumar1*
1Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Chennai - 603203, India.
2Department of Chemical Engineering, SSN College of Engineering, Chennai, India.
*Corresponding Author

Published In:   Volume - 4,      Issue - 4,     Year - 2013


ABSTRACT:
Growing utilization of enzymes in different industries for bioremediation of xenobiotics encourages the search for high bene?t/cost ratio immobilization techniques. Extremely simple procedures for the rapid preparation of magnetic nanoparticle enzyme carriers have been developed and used for the immobilization of laccase as model enzyme. In this study, magnetic nanoparticles (MNP) are used in order to obtain a versatile supporting material for enzyme immobilization. MNP size and morphologies were characterized by scanning electron microscopy (SEM) with energy dispersive X-ray analysis (EDAX). The magnetically separable nanoscaled enzyme catalysts show decreased mass-transfer resistance and improved operational and storage stability. It should be emphasized that the main advantage of this versatile carrier is the possibility of its recovery with effective separation (even up to 5 s) from the reaction mixture with the use of magnet. This magnetic nanobiocatalyst rapidly decolorized 41-96 % of remazol brilliant blue R (RBBR) initially at 50 ppm at 20oC and pH 7.0, when 0.2 U/ml of magnetic laccase was used. UV-Vis spectroscopy analysis confirmed the biotransformation of dyes into several degraded products. The properties of magnetic nanobiocatalysts allow for better optimization and may reduce the total costs of the decolorization of dyes.


Cite this article:
Dandayudham Ramya, Kaushik Raman Varsha, Venkatesh Ranjitha, P. Senthil Kumar, Vaidyanathan Vinoth Kumar. Magnetic Nanoparticles as Versatile Carriers for Immobilization of Laccase. Research J. Engineering and Tech. 4(4): Oct.-Dec., 2013 page 279-283.

Cite(Electronic):
Dandayudham Ramya, Kaushik Raman Varsha, Venkatesh Ranjitha, P. Senthil Kumar, Vaidyanathan Vinoth Kumar. Magnetic Nanoparticles as Versatile Carriers for Immobilization of Laccase. Research J. Engineering and Tech. 4(4): Oct.-Dec., 2013 page 279-283.   Available on: https://ijersonline.org/AbstractView.aspx?PID=2013-4-4-31


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